The Role of CD 34 and D 2-40 in the Differentiation of Dermatofibroma and Dermatofibrosarcoma Protuberans

(Turk Patoloji Derg 2017, 33:223-227) INTRoduCTIoN Dermatofibroma (DF) is a benign fibrohistiocytic tumor usually seen in women and located in the limbs. Dermatofibrosarcoma protuberans (DFSP) is a fibrohistiocytic tumor with intermediate malignant potential. The recurrence rate and the metastatic potential is higher in DFSP. Due to the differences in treatment and prognosis, it is important to diagnose these lesions correctly. In practice, CD34 and Factor XIIIa markers are often used to distinguish DP from DFSP (1, 2). Some studies have reported focal positivity with CD34 in DF, especially in the peripheral region of the lesion (3). D2-40, a monoclonal antibody usually known as podoplanin, is the main marker of lymphatic endothelium. Various studies have stated the rate of D2-40 expression as 0-50% for DFSPs and 86-100% for DFs (4-7). In this study, we aimed to determine the expression of CD34 and D2-40 in DFs and DFSPs together with the possible use of D2-40 as a second step marker after CD34 in differential diagnosis. MATERIAl and METhod

manual immunohistochemistry procedure was performed with D2-40 (Monoclonal Mouse Anti-human, clone D2-40, DAkO) and CD 34 (Monoclonal Mouse Anti-human, clone QBend 10, DAkO) antibodies.Internal vascular structures were accepted as positive control for CD34 and mesothelioma was used as the external positive control for D2-40.Cytoplasmic staining for CD34 and membranous and cytoplasmic staining for D2-40 were accepted as positive and the expressions were classified as follows: no staining was negative; 1-25% of the cells staining, including patchy and peripheral staining, was focal positive; and over 25% the cells staining was diffuse positive.
Statistical analysis was carried out with the SSPS 14.0 software (SPSS, Inc., Chicago, uSA).The statistical associations between CD34 and D2-40 expression in the histological samples were assessed using the chi-squared test.Clinicopathological variables were assessed with the chi-squared and Fisher exact tests.A p value of less than 0.05 was considered to be statistically significant.

RESulTS
The average of age in the DF and DFSP cases was 37.36 and 42.86 years, respectively.In the DF group, 22 (73.3%)cases were female and 8 (26.7%) were male.The male patients formed 60% of the DFSP cases with 9 cases.The localizations of the DF was the limbs in 70%, the trunk in 23% and the head and neck region in 7%s.DFSPs were located on the trunk in 66.7%, head and neck region in 20%, and the limbs in 13.3%.The average diameter of DFs and DFSPs were measured as 0.9 and 5.03 cm, respectively.no significant relationship was found between the DFs and DFSPs according to age distribution (p=0.245).However, there was a significant correlation for sex, localization and diameter of the lesion between the two entities (p=0.03,p=0.002, p=0.001).eleven of 15 DFSPs had follow-up in our study and only one of these had a lung metastasis in the two years after the diagnosis.epidermal hyperplasia and Grenz zone were more frequent in DFs whereas the infiltration of lipomatous tissue and mitosis were usually observed in DFSPs (Figure 1A,B).Among the DF cases, 6 could be subclassifed as the atrophic type in 3, the cellular type in 2 and the lipidized type in 1 whereas 24 were unclassified.One of the 15 DFSP cases showed fibrosarcoma-like and 2 showed malignant fibrous histiocytoma-like areas.In addition 2 DFSPs were associated with myxoid changes.
Seven DF cases expressed CD34 only focally while diffuse CD34 expression was observed in 14 (93.3%) of the DFSPs (Figure 1C).none of the DF cases showed diffuse expression of CD34.Focal positivity was mostly in the peripheral region or in central areas but patchy (Figure 2A).The atrophic type (3 cases), cellular type (2 cases) and lipidized type (1 case) had no CD34 expression.CD34 positivity was lower in the myxoid and sarcomatoid differentiated areas of DFSPs (Figure 2B).Six of the 30 (20%) DFs showed focal and 23 cases (76.7%) showed diffuse D2-40 expression (Figure 2C).Of the 15 DFSPs, only 3 (20%) showed focal and 2 (13.3%) showed diffuse positivity with D2-40.A significant difference was found between the CD34 and D2-40 positivity of the DFs and DFSPs (p=0.000,p=0.000) (Table I).dISCuSSIoN Dermatofibroma (DF) and Dermatofibrosarcoma protuberans (DFSP) are fibrohistiocytic tumors with unknown histogenesis (1).DFs are generally seen in females with a limb localization and a mean diameter of 1 cm while DFSPs are more common in males with a trunk localization and a mean diameter of 5 cm (1,8,9).In our study, the mean age of DFs and DFSPs were not statistically significantly different and were concordant with the literature.DFs and DFSPs had a sex distribution, size and localization similar to that reported in the literature (1,2).DFSP has a local recurrence rate of 7% after extensive resection with negative margins.Its metastasis rate is 5%, most commonly to the regional lymph nodes and lung (1,2).We followed-up 11 DFSP cases in this study and only one had lung metastasis in the two years after the diagnosis.

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We also found that histopathologic features such as a Grenz zone and epidermal hyperplasia were more common in DFs, as reported by korkolis et al. (10).
CD34 is a 115 kD cell surface glycoprotein expressed by myeloid and lymphoid progenitors and endothelial cells (8,11,12).In our study, CD34 positivity was observed in 23.4% of DFs and was only focally positive in the peripheral region of the lesion.However, 14 of the 15 DFSPs (93.3%) showed diffuse CD34 positivity, as in the findings reported by Reimann and Fletcher (13) and Raica et al. (14).The focal positivity in one case could be related to the extensive myxoid change seen in that case.
D2-40 (podoplanin) is a monoclonal antibody of sialoglycoprotein and is used as a lymphatic endothelial marker in determining lymphatic invasion of solid tumors.Tumors with D2-40 expression include lymphangiomas and kaposi sarcoma, ependymoma, meningioma, chondroid tumors and seminomas (14).
The results of studies on D2-40 expression in soft tissue tumors have shown that follicular dendritic cells and tumors originating from these cells express D2-40.However, there is limited data on the expression of D2-40 in DF and DFSPs (4)(5)(6).The more frequent expression of D2-40 in DFs in our study is concordant with reports suggesting a dermal dendrocytic origin of the lesion.In addition, D2-40 was expressed less in DFSPs, suggesting that the DFSP does not originate from dermal dendrocytes and does not contain cells epithelial-mesenchymal transition (6).
Bandarchi et al. have stated that all DFs showed D2-40 with 100% negativity in DFSPs and recommended D2-40 as a helpful marker in differentiating these two lesions (4).In addition, kaddu and leinweber suggested that D2-40 expression could be used in differentiating cellular neurothekeomas and DFs from similar entities in their study on 30 DFs and 15 cellular thekeomas (5).Our results also showed a statistically significant relationship between the D2-40 expressions of DFs and DFSPs.However, a controversial result from the Xu et al. study revealed that many malignant or benign subtypes of soft tissue tumors may show D2-40 expression with no statistically significant specificity (6).
In conclusion, DFs and DFSPs may show similar morphological and immunohistochemical features causing problems in the differential diagnosis, especially in cases without adequate sampling of the lesions.Additional immunohistochemical markers may be needed in DFs with CD34 positivity or DFSPs with myxoid degeneration and sarcomatous differentiation where the CD34 positivity decreases.Our results showed that the higher positivity of D2-40 in DFs compared to DFSPs indicates an additional helpful role for this marker in the differential diagnosis of problematic cases.Further studies are needed to prove the importance of D2-40 in fibrohistiocytic tumors and especially DFs because of the controversial results reported in the literature.In addition, more studies are needed to enlighten the histogenesis of these tumors which may be categorized by their origin in the future.