Gereç ve Yöntem: Klinik olarak lokalize prostat karsinomu nedeniyle prostatları çıkarılan 207 radikal prostatektomi materyalinin vezikülo seminalislerinde amiloid birikimleri değerlendirilmiştir. Amiloid birikimleri Kongo kırmızısı boyama ve polarizasyon mikroskobu ile doğrulanmıştır.

Bulgular: Olguların 10'unda (% 4,8) seminal vezikül amiloidozu saptanmıştır. Hastaların ortalama yaşı 66,2'dir. Amiloid birikimleri, etkilenen seminal veziküllerin epitel altı bölgesinde nodüler ve iki taraflı olma eğilimindedir. Vezikülo seminalislerdeki kan damarları veya prostat parankiminde amiloid birikimleri saptanmamıştır.

Sonuç: Lokalize seminal vezikül amiloidozu alışılmadık bir bulgu değildir. Bu çalışmaya dahil edilen Türk hastalardaki seminal vezikül amiloidozu insidansı ve bu hastaların histopatolojik özellikleri diğer çalışmalardan farklı değildir. Ülkemizde sistemik AA amyloidosis, amiloidozların en yaygın formudur. Seminal vezikül amiloidozu'nın farkında olmak, amiloidozların diğer formlarından ayırımında önemlidir.

Detecting seminal vesicle metastasis during radical prostatectomies performed for prostate adenocarcinomas is quite important for staging. VSA is generally found during evaluation of the samples. Many authors feel that VSA found in radical prostatectomy material is an incidental finding (8- 10). Pitkanen et al. have reported a VSA incidence of 21% in men aged over 75 and have stated that this incidence is due to the aging process and not related to systemic amyloidosis or clinical symptoms⁶. However VSA can also lead to clinical findings such as hematospermia^11-13. Thickening due to accumulation in the seminal vesicles can also be interpreted as tumor invasion on magnetic resonance (MR) imaging and lead to incorrect staging of the prostate tumor^14,15. The relationship with prostate cancer and effect on tumor invasion of amyloid accumulation in the seminal vesicle is unknown.

At least 25 different types of amyloidoses with different etiologies and related prognoses have been defined¹. Semenogelin I, has been defined as the amyloid protein that accumulates in VSA. Spectrometric analyses have revealed that this amyloid fibril consists of polypeptide fragments and these fragments are identical to the N-terminal region of the main secretion of the seminal vesicles¹⁶.

The incidence of the hereditary disorder Familial Mediterranean Fever (FMF) and the related systemic AA amyloidosis is higher in our country than in others. AA amyloidosis is therefore frequently considered in amyloidosis cases. The aim of this study was to determine the amyloidosis frequency and evaluate its pathological characteristics in material obtained from radical prostatectomy performed for prostate adenocarcinoma.

Samples containing the seminal vesicles were routinely stained with Congo Red as well during the study. Puchtler's Alkaline Congo Red technique was used for Congo Red staining^17,18. The slides were evaluated with light and polarized light microscopy and accumulations that stained with Congo Red and showed apple green reflection on the polarized light microscope were accepted as amyloid deposits. The subepithelial region, vessel walls and prostate parenchyma were also evaluated regarding amyloid accumulation. The clinical features of the cases have been collected from the pathology archive records.

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Figure 1: Apperance of nodular amyloid deposits with a subepithelial location in the seminal vesicle on light microscopy (H&E, x40).

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Figure 2: Amyloid deposits that are amorphous and pink-red with Congo Red in the seminal vesicle on light microscopy (Congo Red, x40).

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Figure 3: Appearance of amyloid deposits with apple green reflection on polarized light microscopy (Congo Red, polarized microscope, x40).

The mean age of the VSA cases was 66.2±4.1 years.

The Gleason grades of the prostate carcinoma cases was 6/10 (3+3) in 62 cases, 7/10 (3+4) in 100 cases, 7/10 (4+3) in 39 cases, 8/10 (4+4) in 2 cases, 9/10 (4+5) in 3 cases and 9/10 (5+4) in 1 case. Table I presents the distribution of cases with and without VSA. A tertiary pattern was present in 41 cases. The pathological stage was 2a in 22 cases, 2b in 8 cases, 2c in 113 cases, 3a in 48 cases and 3b in 16 cases. The pathological stage was 2c in 7, 2b in 2 and 3a in 1 of the 10 cases where amyloid deposits were found. Seminal vesicle invasion and/or metastasis was not found in any case with amyloid deposits.

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Table I: Distribution of the cases by Gleason Score

The amyloid that collects in the tissues is amorphous pink on hematoxylin eosin staining while Congo Red staining and polarized light microscopy evaluation differentiates them from other proteinaceous deposits by the apple green birefringence. Amyloid deposits are fibrillar and consist of 8-12 nm fibrils with 8-folded layering on electron microscopy. Amyloidoses can be systemic or localized. Localized amyloid deposits can be as small as a single focus on microscopy or as large as to create a nodular mass. Localized amyloidosis has been defined in many organs^19-24. Localized amyloidoses are thought to be amyloid deposits derived from the proteins synthesized at the cells located in the region of accumulation¹. Localized amyloidosis can be seen together with neuroendocrin tumors (Acal, related to calcitonin) in surgical material. The amyloid protein that accumulated in localized VSA is named “Semenogelin I” (ASem). Linke et al. have demonstrated that Semenogelin I consists of polypeptide fragments and these fragments are identical to the N-terminal region of the main secretion produced by the seminal vesicle¹⁶. It is suggested that localized amyloidosis histogenesis is via the accumulation of the exocrine secretory product of the lining epithelium of the ejaculatory ductus system in the subepithelial seminal vesicle, vas deference and ejaculatory ducti by local diffusion^7,16.

On histopathologic evaluation, the subepithelial location of the seminal vesicle deposits without observing any in the parenchyme or vessel walls is the most important feature differentiating VSA from the systemic amyloidoses. We observed these same findings as in previous studies and they guided us in the VSA diagnosis.

The incidence of VSA in prostatectomy material performed for prostate adenocarcinoma in this study was lower than in the autopsy series in the literature but was consistent with the 4.7% rate reported by Kee et al. for prostatectomy material⁷. Kee et al. have found a difference between two institutions that took care of American and Korean patients as in this study⁷. Although the incidence is similar in the two studies, having multicenter studies with a larger number of cases when we take the between-institution incidence variability into account would enable reaching a more balanced decision.

In contrast to the Pitkanen et al. study, the mean age of the cases with localized amyloidosis was quite similar to that of other cases⁶. We found no relationship between age and the accompanying prostate adenocarcinoma pathological stage with the amyloid accumulation factors such the Gleason score and grade of the tumor and patient age are also effective in the clinical selection criteria of prostate cancer patients at present and we therefore did not undertake statistical analysis.

There are only a limited number of studies on the effect of localized amyloid deposits in the seminal vesicle on the invasion of prostate adenocarcinoma to this region. None of the patients with amyloid deposits in our study had seminal vesicle invasion. It may also be important to evaluate the relationship between the tumor and amyloid deposition as with other amyloidoses. Examples of other amyloidoses are the concurrence of AA amyloidosis and renal tumor, AL amyloidosis and plasma cell tumor for systemic forms and ACal amyloidosis and neuroendocrine carcinoma for localized forms. There are also some cases that have reported a malignancy with the defined ALect2 amyloidosis²⁵.

The differentiation of localized amyloidoses from systemic amyloidoses is quite important due to the different treatment. The first thing that comes to mind for amyloidosis is systemic AA amyloidosis in our country due to the frequency of FMF²⁶. We did not consider systemic amyloidosis in our cases in light of the literature data as there were no vascular amyloid deposits. We did not use commercial immunohistochemical markers against Semenogelin I for typing amyloid as they increase the cost despite the low number of cases. The VSA lesions were positive for lactoferrin and amyloid B protein. No positive reaction was found with antibodies against amyloid A, kappa and lambda and Beta 2 microglobulin¹¹.

We found localized VSA that was consistent with series in cases operated on for prostate adenocarcinoma. This finding can create a suspicion of invasion on MR imaging and needs to be differentiated from systemic amyloidoses that require different treatment protocols and should therefore always be kept in mind by uropathologists. The association of this entity, not commonly seen in prostatectomy material, with tumors should be investigated in wider series.

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