The Role of EGFR , Hepatocyte Growth Factor Receptor ( c-Met ) , c-ErbB 2 ( HER 2-neu ) and Clinicopathological Parameters in the Pathogenesis and Prognosis of Chordoma

Chordoma is a rare malignant bone tumor originating from notochordal remnants (1,2). The complete resection of this tumor is usually not available because of its invasiveness to adjacent structures. although radiotherapy and gamma knife surgery have been tried for treatment, local control cannot be provided in most of the cases due to the extensive recurrence ability of the tumor (3). There are few studies investigating the effect of treatment modalities on the survival and prognosis of chordoma patients (3,4). Histopathological properties of this tumor such as mitosis and necrosis have been analysed in a number of studies in order to understand their relations with the prognosis and survival. However limited information is available from these studies and also the sample sizes are small because of the rare incidence of chordoma. There are also discordances between the results of different studies. Despite several proposed prognostic parameters, no evidence-based parameter has been defined to predict survival (5-7)

tyrosine kinases, resulting in the transphosphorylation of tyrosine residues in the C-terminal regulatory domain.These provide docking sites for downstream molecules and lead to the evasion of apoptosis, and to proliferation, invasion and metastases.Mutation in the tyrosine kinase domain of the eGFr gene was found in a subset of lung cancers (8).erbB2 activation also occurs in a ligand-independent manner, particularly when the receptor is found to be mutated or overexpressed (9).amplification and overexpression of the proto-oncogene erbB2 are found in approximately 15 to 20% of all invasive breast cancers (10).
The c-Met proto-oncogene is located on chromosome 7q21-31 (11).The ligand for c-Met is hepatocyte growth factor (HGF), also known as scatter factor (sF) (12).HGF acts as a cytokine, promoting cell proliferation, survival, motility, scattering, differentiation and morphogenesis (13).recent studies have revealed that the molecular treatments against eGFr, erbB2 and c-met succeed in cases of lung and breast carcinomas and glioblastomas, respectively (14,15).Based on the molecular biology of chordomas, targeted therapy alternatives have been developed (16)(17)(18).However, only a few case reports are found in the literature regarding this issue.
Chordomas show dual expression of epithelial and mesenchymal proteins (2).eGFr, c-Met and c-erbB2 overexpressions have been demonstrated in a majority of malignant tumors (14,15,(19)(20)(21). receptor tyrosine kinases are the novel candidate molecular markers to shed light on the pathogenesis of chordoma.
The aim of this study was the evaluation of histopathological properties such as tumor, type, mitosis, necrosis pleomorphism, hyperchromatic nuclei, apoptosis and presence of mucin, and the immunoexpression patterns of eGFr, c-Met and c-erb-B2, in order to understand their role in the pathogenesis, prognosis, recurrence and survival rates of chordoma.

MATeRIAL and MeTHoDs
Forty-nine skull base and non-skull base chordomas were included in our study in order to analyze the expressions of eGFr (c-erbB1), erbB2 and c-Met by the streptavidinbiotin horseradish peroxidase method (table I).The expression levels of these antibodies and their correlations with the histopathological properties of chordoma such as pleomorphism, mitosis, necrosis, hyperchromasia, extracellular mucin deposition and apoptosis (table II) were evaluated together with the clinical follow-up data including the patient's age, sex, recurrences and overall survival status.
Clinical information and follow-up data were obtained from the neurosurgery Department and telephone interviews of the patients or family members were conducted.recurrence was defined as the relapse of disease that occurred more than 6 months after the initial surgery.Overall survival data was dependent on the current status of the patients as being dead or alive.since autopsies were not performed in cases of death, disease-dependent death interpretation was not available.
all Hematoxylin & eosin (H&e)-stained slides were reviewed by two pathologists for the confirmation of the Quantity statistics (Mean, standard deviation, frequency) and qualitative data were analysed.normally distributed parameters between groups were analyzed with student's t test; and the parameters that were not normally distributed between the groups were analyzed using the Mann-Whitney U test.Quantitative data were assessed with the chi-square test and Fisher's exact chi-square test.survival analysis was evaluated with kaplan-Meier survival analysis excluding those lost to follow up or who died due to postoperative surgical complications.log rank (mantel-Cox) analysis was used to compare the prognostic significance of the antibodies' expressions on survival.results were evaluated using a 95% confidence interval and significance was considered when the p value was lower than 0.05.

Patients and Follow up
We diagnoses.at the time of initial diagnoses of the cases, immunomarkers such as epithelial membrane antigene (eMa), cytokeratin, vimentin, s100 and glial fibrillary acidic protein (GFaP) had been applied as well as the histological criteria of chordoma for the exclusion of other malignancies such as adenocarcinomas.Pas-alcian Blue stain had been used for extracellular mucin identification.none of the cases had a concomitant malignancy.
Hyperchromasia was noted when more than 50% of the nuclei were intensely hyperchromatic.
Pleomorphism was noted when major cellular differences were detected in more than 50% of the tumor cells.
at least ten representative fields were analysed at high magnification (x400 objective) for determining mitosis.
all fields were examined to detect necrosis and any ratio of detectable necrosis was noted when seen.
This study was approved by the ethics Committee of the relevant institute with the identifying code Mar-yC-2008-0213.an informed consent form regarding permission for clinical research was obtained from each patient.

Immunohistochemistry
Four-micrometer-thick sections were placed on 3-aminopropyletxylene-covered slides and deparaffinized at 60 ºC for 1 h.The slides were dewaxed in xylene and dehydrated in 96% alcohol.sections were then immersed in 10 mmol⁄ l citrate buffer (pH 6.0) and microwaved for 20 min for antigen retrieval.slides were cooled to room temperature and rinsed in phosphate-buffered saline (PBs).endogenous peroxidase activity was blocked by further pretreatment with 3% H2O2 ⁄ distilled water for 20 min at room temperature.after a thorough washing in PBs, blocking solution (lab Vision Co., Fremont, Ca, Usa) was applied to block nonspecific antibody binding.
after a 10-min rinse in PBs, biotinylated goat antipolyvalent immunoglobulin (lab Vision Co., Fremont, Ca, 94539) for 20 min, and in the streptavidin-biotin peroxidase complex for 20 min being applied at rt, 3,3¹ diaminobenzidine chromogen was used for visualization of antigen-antibody binding.The sections were counterstained with hematoxylin, dehydrated, cleared in xylene and mounted on entellan coverslips.
two cases showing mitotic activity were dedifferentiated and conventional chordomas respectively.Mitotic activity had been 4/10 HPF (High power field) in the dedifferentiated variant and 2/10 HPF in the conventional variant.
Three of 6 cases with necrosis were conventional chordomas while two were chondroid and the remaining one was dedifferentiated chordoma.The highest extension ratio of necrosis (necrotic area/total tumor area) was 50% while the lowest ratio was 10%.
two of the 4 cases showing pleomorphism had chondroid morphology while the others had been dedifferentiated and conventional chordomas.The case with dedifferentiated histology revealed marked pleomorphism and atypical features such as sarcomatoid areas, high nuclear/ cytoplasmic ratios and irregular nuclear membranes.Mild pleomorphism was present in the other three cases.
One patient had 5 recurrences, 1 patient had 3 recurrences, 2 patients had 2 recurrences while the other 13 patients had one recurrence.The mortality rate was 50% as 20 patients died.among all cases, localization was cranial in 40 and spinal in 9 patients.

Histopathological evaluation
according to the WHO classification; 40 tumors were of the conventional type (Figure 1), 8 were chondroid (Figure 2) and 1 was dedifferentiated (Figure 3).The dedifferentiated histology was present in the initial biopsy of the patient.The case with dedifferetiated histology had been nonrecurrent after being followed for 84 months.
On histopathological examination, mitosis was found in 2 cases, necrosis in 6 cases (Figure 4), pleomorphism in 4 cases, and hyperchromatic nuclei in 15 cases.apoptosis was not observed in any of the cases and all cases showed mucin deposition (table II).
The only significant statistical correlation among histopathological parameters was between necrosis and survival rates (table III, Figure 5).survival rates were lower in cases with necrosis (p<0.01).no correlation was detected between histological features and prognosis (recurrence and overall survival).

expression of eGFR, c-Met and c-erbB2
Immunohisto-chemical evaluation revealed that 77.5% of the cases (38/49) were intensely positive (3+) by eGFr (Figure 6) and 12.2% (6/49) by c-Met (Figure 7).two cases were negative by eGFr and 34 were negative by c-Met.none of the cases stained by c-erbB2.The only significant correlation was found between c-Met expression and recurrence.recurrence rates were higher in c-Met intensely positive cases (p<0.05)(table IV).no significant correlation was found between immunoexpression profiles and clinicopathological parameters including age, sex, localization of tumor, histological type and the histopathological properties of the tumor.

DIsCussIon
although studies based on the correlation of prognosis and histopathological parameters such as mitosis, apoptosis and extracellular mucin in chordoma are present in the literature, no definite evidence-based histopathological adverse prognostic factor predicting recurrence has been found (22,23).Chordomas are believed to originate from mesenchymal tissue; however they have recently been considered to have dual epithelial and mesenchymal differentiation (2).receptor tyrosine kinases such as eGFr, c-Met ve c-erbB2 are implicated in the pathogenesis of various epithelial tumors.The molecular-targeted therapies have been recently administered to patients whose tumors have tyrosine kinase activity (15,19,21).In our study, a cranial localization (81.6% of all cases) was the most frequent site.This finding contradicts the previous studies    eight cases (16.3%) in our study were diagnosed as chondroid chordoma.This finding is compatible with the most comprehensive study of chordoma in which 22 of 155 (14%) cases were diagnosed as chondroid chordoma (6).
In a study based on chondroid chordoma, survival rates were found to be better in chondroid chordoma compared with classical chordoma (6).In another study, no specific correlation was found between the histological type of chordoma and prognosis (28).In our study, we have not found a statistically significant correlation between the histological type and prognosis of chordoma.
necrosis and survival rates have been found to be correlated with each other.survival rates are worse in the cases in which necrosis is reported.This result is consistent with the two other studies in the chordoma literature (28,31).However, there are other studies claiming that necrosis has no role in chordoma prognosis (22,32).
no statistical correlation was found between the other parameters (mitosis, pleomorphism, presence of mucin) and prognosis.
In the study of Bjornsson et al. chondroid differentiation and cellular atypia were investigated and these parameters were not correlated with the biological behaviour of chordoma (32).Histopathological features such as hypercellularity, pleomorphism, apoptosis, necrosis and proliferative index by ki-67 antibody were assessed in in which the sacrococcyx is presented as the most frequent site (24,25).The reason of this contradiction may be the sample size of our study.
Chordoma predominantly affects men aged 40-50 years (5,(24)(25)(26).In our study, the mean age of the patients was 40.9± 18.05 years and 63.3% of the patients were male.In a study of 51 cases, survival rates were better in patients younger than 40 years regardless of the tumor's histopathological features, and age was defined as an independent factor (27). O'Connel et al. proposed female gender as a poor prognostic indicator in their study of 62 patients (28).
The most effective treatment for chordoma is still surgery.Complete resection or gross total resection chance depends on the localization of the tumor.residual chordoma deeply infiltrating the surrounding tissues mainly account for the local recurrences.For complete tumor removal, a radical wide posterior surgical margin should be employed while dealing with sacrococcygeal cases (26).McGirt et al. assessed 67 sacral and 47 mobile spine chordoma patients who had been surgically treated and revealed that sacral localization was associated with poor survival in chordoma (29).In another study based on 29 cases, vertebral localization appeared to be a significant risk factor for recurrence (30).In a study by Fasig et al., no correlation was found between tumor localization and recurrence in 20 chordoma cases (16).We have not found a specific correlation between localization and recurrence in our study.eGFr and c-Met were found to be expressed in 75.0%, 83% and 77% of primary lesions.In the subsequent study of akhavan-sigari et al. positive immunostaining with eGFr and c-Met was detected in 92% and 100% of 145 tumor specimens (34).Fasig et al. studied receptor tyrosine kinases in 21 chordoma cases and found PDGFr-b, eGFr, kIt and c-erbB2 expressions in 100%, 67%, 33% and 0% of cases, respectively (16).naka et al. investigated HGF/c-Met expression in 46 primary and 25 recurrent lesions and found c-Met expression in 70.0% of primary and 88.0% of recurrent lesions (35).eGFr expression has been reported in 67-100% and c-Met expression varies between 70% and 100% in these corresponding studies.In our study, eGFr and c-Met expression rates were 77.6% and 12.2%.The c-Met expression rate is lower than the rates given in the studies mentioned in the literature.This discrepancy with the literature could be associated with the clone of the antibody used in our study which differed from the clone and brand of c-Met antibody that had been used in the regarding studies.This discordance could also be explained by the sample size.Further molecular research as well as immunohistochemical studies with a larger number of cases are indicated in order to analyse the expression rate of c-Met in chordoma.Peghini et al. demonstrated positive correlation with eGFr and c-Met staining in gastrinomas and the staining was also related with the aggressive behaviour of the tumors (36).
Pharmacological inhibition of eGFr kinase has no impact on the blockage of c-Met activity, however this inhibition abolishes the proliferative effect after HGF binds to its receptors (37).Common downstream pathways between eGFr, HGF and c-Met may explain this mechanism.a study of 21 chordoma cases analysing the genes in the downstream pathways of eGFr including p44/42 MaPk, akt, stat3 has revealed that 67% of the cases were reactant by eGFr while the phosphorylated forms of p44/42 MaPk, akt, stat3 were expressed in 86%, 76%, and %67 respectively.The high expression profiles of these genes strongly indicate a role for thyrosine kinase activity in the pathogenesis of chordoma (16).Microarray studies analysing multiple genes are required to examine the exact mechanism of eGFr, cMet and c-erbB2 in chordoma.
The c-Met protooncogene is located in chromosome 7q31.21.scheil et al. showed 7q gain in 69% of chordomas (38).c-Met expression via 7q amplification may be a boarding step of chordoma progression.c-Met and HGF are reported to be implicated in chordoma etiology and metastatic progression (39).
another study.tumor growth was found to be increased in cases showing high mitotic ratios and a proliferative index more than 6% (22).Mitosis was reported only in two cases in our study.no statistically signficant correlation was shown between cases with mitotic activity and prognosis.The controversial results of the studies in the literature can be explained by the low number of the cases due to the rarity of chordoma.
a limitation of our study is the correlation of survival and prognosis with the proliferative index shown by ki-67 antibody.We have used the residual tissues in the paraffin blocks of the patients after the routine reporting process.During the reporting process, almost all of the cases were evaluated not only by H&e stain but also immunohistochemical stains such as eMa, s100, GFaP, vimentin and Ck.The majority of cases included in our study have been intracranial chordoma cases with limited tissue samples with volumes lower than 1 cm³.after the preparation of the routine H&e stain, histochemical Pas-alcian Blue stain, sections were taken for the immunohistochemical analysis of three receptor tyrosine kinases.Immunohistochemical ki-67 application was available in 10 cases and the proliferative activities were less than 1% in 6 cases, 2% in two cases, 3% in one case and 5% in one case.
Immunohistochemical staining revealed that 77.6% of the cases were intensely positive (3+) by eGFr and 12.2% by c-Met.none of the cases stained by c-erbB2.a statistically significant correlation was found between c-Met expression and recurrence.recurrence rates were higher in patients with high levels of c-Met expression.a positive correlation was determined between extension of staining by eGFr and c-Met.eGFr positive cases showed significant c-Met immunoexpression (p<0,01).
Weinberger et al. examined the expression of c-Met, c-erb-b2 (Her2/neu), and epidermal growth factor (eGFr) in a cohort of 12 chordomas and found that all chordomas had positive expressions of both the hepatocyte growth factor/scatter factor receptor and eGFr.c-erbB2 expression was also detected in 7 out of 12 cases.another outcome of this study was the positive correlation between eGFr and c-Met expressions.Chordoma tumors that had high c-Met expression were also likely to have high eGFr expression (17).
In a study by akhavan-sigari at al. using immunohistochemical techniques, the authors investigated Plateletderived growth factor receptor-α (PDGFr α), eGFr and c-Met expression in 52 primary lesions (33).PDGFr-α, recurrence rates were higher in cases with intensely positive c-Met staining.all of the 4 cases with high c-Met expression showed recurrences.One of them showed two recurrences and three showed a single recurrence.localization was cranial in all cases and gross total resection was performed in each case.two of the cases died at the 33rd and 45th months while the others were alive.Follow-up periods for the latter were 96 and 168 months after surgery.Three of these cases were conventional while one was chondroid.Hyperchromatic nuclei were evident in a single case and the others did not show any atypical histological feature at the time of initial biopsies.
There are studies in the literature revealing the correlation of c-Met staining and poor prognosis in glioblastoma, bladder, cervix, lung and liver neoplasms and multiple myeloma (40).In the study of akhavan-sigari et al., recurrent chordoma lesions with higher c-Met expression were found to be associated with significantly better prognosis than those with lower c-Met expression.Those patients with lower eGFr expression were found to have significantly better prognosis than those with higher eGFr expression (33).However, in our study, recurrence rates were high in intensely c-Met positive cases.This finding could also be explained by the limited sample size of the studies.Therefore meta-analysis reports should be prepared to obtain more reliable data about the correlation between the histological features and immunostaining profiles of chordoma and prognosis.none of our cases showed immunoreactivity by c-erbB2.
Conflicting results are present comparing the two other studies investigating c-erbB2 in chordoma.Weinberger et al. claim that c-erbB2 immunoreactivity is seen in 7 cases out of 11 (17) while in the study of Fasig et al no reactivity by c-erbB2 is demonstrated although two different clones of this antibody being used (16).Our results are consistent with the study of Fasig et al.
Complementary studies such as flourescence in situ hybridization and Western Blot analysis with more cases are needed to argue whether c-erbB2 has a role in the pathogenesis of chordoma.
Because of the aggressive potential and poor prognosis of chordoma, new therapeutical strategies should be investigated.Hof et al. presented a case of chordoma under a combination regimen including cetuximab and gefitinib who showed partial regression in pulmonary metastasis (18).c-Met expression could be used as an indicator of recurrence risk, as a target for therapeutic agents to decrease recurrence, and for the prediction of prognosis.

Figure 5 :
Figure 5: the kaplan-Meier survival curves of two groups of cases according to presence of necrosis.

Table I :
Characteristics of patients with chordoma * Follow-up was available in 40 cases out of 49.

Table II :
Histopathological parameters of the cases

Table III :
necrosis and survival analysis

Table IV :
Correlation of eGFr, c-Met and c-erbB2 profiles with recurrence *Mann-Whitney U test